September 6th, 2010
•In this protocol, we described a new method to study the influence of glial cell heterogeneity on axon growth with an in vitro co-culture system. Rat cortical glial cells were cultured to confluence and cocultured with highly purified rat dorsal root ganglia neurons. Different glial cell influence on neurons adhesion and axon growth was compared directly in the same culture. This method provides a new way to directly study the glial cell heterogeneity influence on neuron adhesion and axon growth.
Related Videos
A Caenorhabditis elegans Model System for Amylopathy Study
A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior
Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
New Methods to Study Gustatory Coding
Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy
Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats
A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved