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Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

DOI :

10.3791/3257-v

November 12th, 2011

November 12th, 2011

19,174 Views

1Department of Pharmacology and Physiology, Drexel University College of Medicine

Here we provide a protocol for culturing rat cortical neurons in the presence of a glial feeder layer. The cultured neurons establish polarity and create synapses, and can be separated from the glia for use in various applications, such as electrophysiology, calcium imaging, cell survival assays, immunocytochemistry, and RNA/DNA/protein isolation.

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Bilaminar Co culture

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