<ol>
<li>Make microinjection needles by pulling glass capillary tubes. We use a needle puller from Sutter Instrument CO, (Model P-2000). The needle puller program specifications are: heat: 275 fil: 3 vel: 37 del: 250 pul: 125 </li>
<li>Load a known volume (e.g., 1 &mu;l) of water in the needles and manually mark the scale with a fine sharpie.</li>
<li>Set up a post-injection cup for the mosquitoes. First cut the outmost tip off of a 1000 &mu;l pipette tip. Then, cut a hole in the side of a cup and ...

injection of dsrna into female a. aegypti mosquitos

Reverse genetic approaches have proven extremely useful for determining  which genes underly resistance to vector pathogens in mosquitoes.  This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus.  The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Watch this Scientific Journal Video about Injection of dsRNA into Female A. aegypti Mosquitos at JoVE.com

Injection of dsRNA into Female A. aegypti Mosquitos

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Reverse genetic approaches have proven extremely useful for determining  which genes underly resistance to vector pathogens in mosquitoes.  This video ...

injection-of-dsrna-into-female-a-aegypti-mosquitos

Research

JoVE Journal

Biology

12.1K 조회수.  University of California, Irvine (UCI). 역방향 유전자 접근 방법은 모기에 벡터 병원균하는 유전자 underly 저항을 결정하는 매우 유용하게 입증했습니다. 이 비디오 프로토콜은 뎅그열 바이러스를 항구 여성 A. aegypti 모기로 dsRNA를 주입하기 위해 제임스 연구실에서 사용하는 방법을 보여줍니다. , 주사 바늘을 보정 사출 설정을 조작하고, 흉부에 dsRNA를 주입에 대한 기술은 그림이다.