Name: injection of dsrna into female a. aegypti mosquitos
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Injection of dsRNA into Female A. aegypti Mosquitos at JoVE.com

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<li>Make microinjection needles by pulling glass capillary tubes. We use a needle puller from Sutter Instrument CO, (Model P-2000). The needle puller program specifications are: heat: 275 fil: 3 vel: 37 del: 250 pul: 125 </li>
<li>Load a known volume (e.g., 1 &mu;l) of water in the needles and manually mark the scale with a fine sharpie.</li>
<li>Set up a post-injection cup for the mosquitoes. First cut the outmost tip off of a 1000 &mu;l pipette tip. Then, cut a hole in the side of a cup and ...

injection of dsrna into female a. aegypti mosquitos

Reverse genetic approaches have proven extremely useful for determining  which genes underly resistance to vector pathogens in mosquitoes.  This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus.  The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Watch this Scientific Journal Video about Injection of dsRNA into Female A. aegypti Mosquitos at JoVE.com

Injection of dsRNA into Female A. aegypti Mosquitos

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Reverse genetic approaches have proven extremely useful for determining  which genes underly resistance to vector pathogens in mosquitoes.  This video ...

Research

JoVE Journal

Biology

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

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