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Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein

DOI :

10.3791/2198-v

9:53 min

September 23rd, 2010

September 23rd, 2010

12,597 Views

1National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology, George Mason University

We have developed a lentiviral vector that possesses, in addition to the Tat-responsive LTR, the Rev-response element (RRE) that can regulate reporter gene expression in an HIV-1 Tat- and Rev-dependent fashion. The vector permits the specific detection of replicating HIV in living cells via the expression of GFP.

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HIV 1

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