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The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

DOI :

10.3791/2259-v

4:10 min

October 12th, 2010

October 12th, 2010

89,559 Views

1Department of Immunology, John Curtin School of Medical Research, Australian National University

CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.

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Carboxyfluorescein Diacetate Succinimidyl Ester

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