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Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells

DOI :

10.3791/2250-v

November 9th, 2010

November 9th, 2010

19,234 Views

1Pathology and Laboratory Medicine, University of Wisconsin-Madison, 2Pathobiological Sciences, University of Wisconsin-Madison

Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.

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