October 16th, 2010
•Our protocol describes how to dissect the rat abdominal mammary gland and how to prepare mammary gland whole mounts. It also describes how to analyze mammary gland morphology using three end-points (number of terminal end buds, epithelial elongation and differentiation) and to use these results to predict mammary cancer risk in rats which were exposed to dietary modifications in utero or during prepuberty.
Tags
Related Videos
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Evaluation of Mammary Gland Development and Function in Mouse Models
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines
Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology
Bovine Mammary Gland Biopsy Techniques
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved