April 7th, 2011
•ITC is a powerful tool for studying the binding of a ligand to its host. In complex systems however, several models may fit the data equally well. The method described here provides a means to elucidate the appropriate binding model for complex systems and extract the corresponding thermodynamic parameters.
Related Videos
Titration of Human Coronaviruses Using an Immunoperoxidase Assay
Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
Culturing Primary Rat Inner Medullary Collecting Duct Cells
Biochemical Titration of Glycogen In vitro
Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved