Lysing and Spreading a Nucleus from a Biopsied Blastomere
2:38
In Situ Hybridization of a Single Blastomere Nucleus
5:50
FISH Diagnosis
7:08
Conclusion
This article describes the selection of suitable probes for single-cell FISH, spreading techniques for blastomere nuclei, and in situ hybridization and signal scoring, applied to pre-implantation genetic diagnosis (PGD) in a clinical setting.