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Mouse In Vivo Placental Targeted CRISPR Manipulation

DOI :

10.3791/64760-v

April 14th, 2023

April 14th, 2023

1,581 Views

1Interdisciplinary Graduate Program in Genetics, University of Iowa, 2Department of Psychiatry, Carver College of Medicine, University of Iowa, 3Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa, 4Hawk-IDDRC, University of Iowa

Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.

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