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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

DOI :

10.3791/58844-v

January 9th, 2019

January 9th, 2019

12,476 Views

1Institute of Microbiology and Immunology, National Yang-Ming University

This article illustrates a powerful method to quantify mitochondria or lysosomes in living cells. The combination of lysosome- or mitochondria-specific dyes with fluorescently conjugated antibodies against surface markers allows the quantification of these organelles in mixed cell populations, like primary cells harvested from tissue samples, by using multicolor flow cytometry.

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Multicolor Flow Cytometry

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