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Step-specific Sorting of Mouse Spermatids by Flow Cytometry

DOI :

10.3791/53379-v

December 31st, 2015

December 31st, 2015

10,272 Views

1Department of Biochemistry, Université de Sherbrooke, 2Department of Medicine, Université de Sherbrooke

We describe a sorting strategy for mouse spermatids using flow cytometry. Spermatids are sorted into four highly pure populations, including round (spermiogenesis steps 1-9), early elongating (spermiogenesis steps 10-12), late elongating (spermiogenesis steps 13-14) and elongated spermatids (spermiogenesis steps 15-16). DNA staining, size and granulosity are used as selection parameters.

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Keywords Spermatid Differentiation

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