Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
4:40
Cell Seeding
5:21
Graft Implantation
8:28
Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
9:27
Conclusion
Transcript
Clinical applications of tissue-engineered trachea have been limited due to graft stenosis and delayed epithelialization. A mouse model of orthotopic tracheal replacement permits the study of the cellular mechanisms driving regeneration. Long-segm
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Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.