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Counting Human Neural Stem Cells

DOI :

10.3791/262-v

August 22nd, 2007

August 22nd, 2007

15,162 Views

1Department of Pathology, University of California, Irvine (UCI)

Knowledge of the exact number of viable cells is required for many tissue culture manipulations. This protocol describes how to differentiate between live and dead cells and quantify cells using a hemacytometer. Although it describes counting human neural stem/precursor cells (hNSPCs), it can be used for other cell types.

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