Immunocytochemistry: Human Neural Stem Cells

27.2K Views

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19:41 min

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August 24th, 2007

DOI :

10.3791/267-v

August 24th, 2007

•

Steven Marchenko¹, Lisa Flanagan¹

¹Department of Pathology, University of California, Irvine (UCI)

Summary

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Immunocytochemistry

Human Neural Stem Cells

Antigen

Protein

Cultured Cells

Subcellular Localization

Chapters in this video

0:00

Title

0:07

Introduction

0:57

Day 1: Preparing German Glass Coverslips and Seeding Cells

3:16

Day 2: Fixing, Permeablilization, and Adding Primary Ab to Cells

12:47

Day 3: Washing, secondary Ab incubation, nuclear stain, and mountin

18:53

Conclusion

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