JoVE Journal

Immunology and Infection

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Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

57.2K Views

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08:01 min

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May 2nd, 2011

DOI :

May 2nd, 2011

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Manira Rayamajhi¹, Elizabeth F. Redente², Tracy V. Condon¹, Mercedes Gonzalez-Juarrero³, David W.H. Riches¹^,²^,⁴, Laurel L. Lenz¹^,⁴

¹Department of Immunology, University of Colorado School of Medicine, ²Division of Cell Biology, Department of Pediatrics, National Jewish Health , ³Department of Microbiology, Immunology, and Pathology, Colorado State University, ⁴Department of Immunology, National Jewish Health

Summary

We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.

Chapters in this video

0:05

Title

1:32

Intratracheal Injection

3:11

Harvesting and Processing Lung Draining Lymph Nodes and Lungs for Flow Cytometry

6:33

Representative Flow Cytometric Analyses of Lung Draining Lymph Nodes After Intratracheal Injection

7:39

Conclusion

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