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JoVE Journal

Immunology and Infection

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'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

13.3K Views

11:31 min

July 8th, 2011

July 8th, 2011

13,302 Views

0:05

Title

2:04

Y. Pestis Plate Inoculation

2:53

Y. Pestis Liquid Media Inoculation

3:30

Y. Pestis Outgrowth Reporter Phage Addition and Bioluminescence Detection

7:06

Single Time Point, Expedited Detection

7:41

Data Analysis and Representative Results

9:54

Conclusion

A simple method for the identification of priority bacterial pathogens is to use genetically engineered reporter phage. These reporter phage, which are specific to their particular host species, are capable of rapidly transducing a bioluminescent signal response to host cells. Herein, we describe the use of reporter phage for the detection of Yersinia pestis.

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