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Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens (Video) | JoVE

DOI :

10.3791/2740-v

July 8th, 2011

July 8th, 2011

13,228 Views

1BioSciences Division, Guild Associates, Inc., 2Department of Molecular Genetics and Microbiology, University of Texas at Austin, 3Department of Craniofacial Biology, Medical University of South Carolina

A simple method for the identification of priority bacterial pathogens is to use genetically engineered reporter phage. These reporter phage, which are specific to their particular host species, are capable of rapidly transducing a bioluminescent signal response to host cells. Herein, we describe the use of reporter phage for the detection of Yersinia pestis.

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