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Extraction of High Molecular Weight DNA from Microbial Mats Video (Video) | JoVE

DOI :

10.3791/2887-v

July 7th, 2011

July 7th, 2011

19,214 Views

1Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina

We provide an improved protocol for extracting high molecular weight DNA from hypersaline microbial mats. Microbial cells are separated from the mat matrix prior to DNA extraction and purification. This enhances the concentrations, quality, and size of the DNA. The protocol may be used for other refractory samples.

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