September 4th, 2011
•Mutations in the kisspeptin receptor (KISS1R) are associated with reproductive disorders in patients. Here we describe how to introduce mutations of interest in the GC-rich sequence of KISS1R as well as the use of KISS1R constructs to characterize the degradation pathway of the receptor by immunoprecipitation and western blot.
Related Videos
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved