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High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

DOI :

10.3791/2942-v

August 13th, 2011

August 13th, 2011

19,997 Views

1Department Of Chemistry and Chemical Engineering, California Institute of Technology, 2Chemistry and Chemical Engineering, California Institute of Technology

We describe a low cost, high throughput method to screen for fungal endoglucanase activity in E. coli. The method relies on a simple visual readout of substrate degradation, does not require enzyme purification, and is highly scalable. This allows for the rapid screening of large libraries of enzyme variants.

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High Throughput Screening

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