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Laser-guided Neuronal Tracing In Brain Explants

DOI :

10.3791/53333-v

November 25th, 2015

November 25th, 2015

8,668 Views

1Department of Physiology and Biophysics, University of Colorado School of Medicine

We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

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Laser guided

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