JoVE Logo
Faculty Resource Center

Sign In

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

DOI :

10.3791/50374-v

June 25th, 2014

June 25th, 2014

64,520 Views

1Department of Nutrional Sciences, University of Wisconsin-Madison, 2Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Wisconsin-Madison, 3School of Pharmacy, University of Waterloo

Assaying in vitro β-cell function using isolated mouse islets of Langerhans is an important component in the study of diabetes pathophysiology and therapeutics. While many downstream applications are available, this protocol specifically describes the measurement of intracellular cyclic adenosine monophosphate (cAMP) as an essential parameter determining β-cell function.

Tags

Pancreatic Islet Isolation

-- Views

Related Videos

article

Murine Pancreatic Islet Isolation

article

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

article

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

article

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

article

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

article

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

article

Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

article

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

article

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

article

A Method for Islet Transplantation to the Omentum in Mouse

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved