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Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

DOI :

10.3791/3149-v

August 17th, 2011

August 17th, 2011

20,797 Views

1Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston

This video provides a detailed protocol for studying the pharmacological profile of human TRPA1 channels using FlexStation 3. The protocol covers details of cell preparation, dye loading and operation of the microplate reader, FlexStation 3.

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Cell based Calcium Assay

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