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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

DOI :

10.3791/51087-v

9:37 min

August 15th, 2014

August 15th, 2014

42,156 Views

1Lawrence Berkeley National Laboratory, The Molecular Foundry

More than half of proteins are small proteins (molecular mass <200 kDa) that are challenging for both electron microscope imaging and three-dimensional reconstructions. Optimized negative staining is a robust and high-throughput protocol to obtain high contrast and relatively high resolution (~1 nm) images of small asymmetric proteins or complexes under different physiological conditions.

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Optimized Negative Staining

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