JoVE Logo
Faculty Resource Center

Sign In

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

DOI :

10.3791/61870-v

May 28th, 2021

May 28th, 2021

3,893 Views

1Huffington Center on Aging, Baylor College of Medicine, 2Department of Molecular and Human Genetics, Baylor College of Medicine, 3Howard Hughes Medical Institute, Baylor College of Medicine

Stimulated Raman scattering (SRS) microscopy allows selective, label-free imaging of specific chemical moieties and it has been effectively employed to image lipid molecules in vivo. Here, we provide a brief introduction to the principle of SRS microscopy and describe methods for its use in imaging lipid storage in Caenorhabditis elegans.

Tags

Stimulated Raman Scattering Microscopy

-- Views

Related Videos

article

Label-free in situ Imaging of Lignification in Plant Cell Walls

article

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

article

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

article

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

article

Basic Caenorhabditis elegans Methods: Synchronization and Observation

article

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

article

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

article

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

article

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

article

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved