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Immunostaining of Dissected Zebrafish Embryonic Heart

DOI :

10.3791/3510-v

January 10th, 2012

January 10th, 2012

17,422 Views

1Division of Cardiovascular Diseases, Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine

A rapid way to conduct immunostaining of zebrafish embryonic heart is described. Compared to the whole mount immunostaining approach, this method dramatically increases the penetration of the antibodies, which allows obtaining high resolution images that reveal cellular/subcellular structures in the heart within a much reduced processing time.

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Immunostaining

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