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JoVE Journal

Neuroscience

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Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers

19.2K Views

14:06 min

June 26th, 2012

June 26th, 2012

19,237 Views

0:05

Title

1:54

Preparation of the Head Restraint

4:14

Stereotaxic Alignment for Craniotomy

8:24

Head-post and Ground Pin Installation

10:20

Craniotomy

11:54

Results: Electrophysiological Recordings and Tracing Studies of the Inferior Colliculus

13:19

Conclusion

Electrophysiological characterization of neuronal responses is important for understanding brain function and for guiding the placement of dyes for pathway tracing. However, many studies are performed in anesthetized animals. To understand brain function without anesthetics, we developed a method to record neuronal response properties and inject dyes in awake mouse.

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