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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

DOI :

10.3791/3764-v

17:38 min

April 27th, 2012

April 27th, 2012

15,300 Views

1Howard Hughes Medical Institute, Department of Neurobiology, Duke University , 2Department of Biological Sciences, Hokkaido University

This protocol is successfully used to quantitatively detect levels and spatial patterns of mRNA expression in multiple tissue types across vertebrate species. The method can detect low abundance transcripts and allows processing of hundreds of slides simultaneously. We present this protocol using expression profiling of avian embryonic brain formation as an example.

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Radioactive In Situ Hybridization

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