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Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

DOI :

10.3791/52799-v

June 17th, 2015

June 17th, 2015

11,523 Views

1College of Physicians and Surgeons, Columbia University

RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.

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Axonally Localized MRNAs

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