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Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

DOI :

10.3791/53976-v

March 26th, 2016

March 26th, 2016

12,586 Views

1Wolfson Institute for Biomedical Research, University College London

Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.

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