Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

10.8K Views

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10:27 min

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March 9th, 2012

DOI :

10.3791/3794-v

March 9th, 2012

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Robert DeRose¹, Christopher Pohlmeyer¹, Nobuhiro Umeda¹^,², Tasuku Ueno¹^,², Tetsuo Nagano², Scot Kuo¹^,³, Takanari Inoue¹

¹Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, ²Graduate School of Pharmaceutical Sciences, University of Tokyo, ³Biomedical Engineering, Johns Hopkins University

Summary

Chapters in this video

0:05

Title

2:17

Transfection of Plasmid DNA

4:02

Preparation of cRb-A Solution

5:22

Microscopy and Light Illumination at the Subcellular Level

7:07

Examining the Effect of RAC Activation on Local Ruffle Formation

9:29

Conclusion

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