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Super-Resolution Live Cell Imaging of Subcellular Structures

DOI :

10.3791/61563-v

January 13th, 2021

January 13th, 2021

4,423 Views

1Department of Biology, The Johns Hopkins University

Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live tissue.

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Keywords Super resolution

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