March 22nd, 2018
•Here, we present a protocol to analyze cell-to-cell transfer of oscillatory information by optogenetic control and live monitoring of gene expression. This approach provides a unique platform to test a functional significance of dynamic gene expression programs in multicellular systems.
Tags
Related Videos
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved