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An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

DOI :

10.3791/57149-v

March 22nd, 2018

March 22nd, 2018

7,477 Views

1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Japan Science and Technology Agency, PRESTO, 3Institute for Integrated Cell-Material Sciences, Kyoto University, 4Graduate School of Medicine, Kyoto University, 5Graduate School of Biostudies, Kyoto University

Here, we present a protocol to analyze cell-to-cell transfer of oscillatory information by optogenetic control and live monitoring of gene expression. This approach provides a unique platform to test a functional significance of dynamic gene expression programs in multicellular systems.

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Keywords Optogenetics

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