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Growth of Mycobacterium tuberculosis Biofilms

DOI :

10.3791/3820-v

February 15th, 2012

February 15th, 2012

23,314 Views

1Department of Infectious Diseases and Microbiology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh

Mycobacterium tuberculosis forms drug tolerant biofilms when cultured in certain conditions. Here we describe methods for culturing M. tuberculosis biofilms and determining the frequency of drug tolerant persisters. These protocols will be useful for further studies into the mechanisms of drug tolerance in M. tuberculosis.

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Mycobacterium Tuberculosis

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