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Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

DOI :

10.3791/1512-v

September 4th, 2009

September 4th, 2009

48,025 Views

1Institute of Biomedical Sciences, Federal University of Rio De Janeiro-UFRJ

Karyotyping is a simple and useful technique widely used for detecting genetic alterations. Here we describe a step by step protocol for chromosome spread preparation of human embryonic stem cells for monitoring the chromosomal status of these cells maintained in culture.

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Chromosomal Spread Preparation

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