Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)
26.2K Views
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14:15 min
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June 18th, 2012
DOI :
June 18th, 2012
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Chapters in this video
0:05
Title
3:11
Generation of a Single-cell Suspension of Leukocytes from Spleens
5:09
Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
9:01
Magnetic Enrichment of Gr-1+ Leukocytes
12:06
Post-sort Analysis of Gr-1-enriched Leukocytes
12:51
AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
13:40
Conclusion
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