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Isolation of Immune Cells from Primary Tumors

DOI :

10.3791/3952-v

June 16th, 2012

June 16th, 2012

36,857 Views

1Tumor Immunity and Tolerance Section, Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, National Cancer Institute - Frederick

In this report, we describe a protocol for isolating highly purified populations of leukocytes that infiltrate tumors. This protocol is adapted from the Miltenyi Biotech protocol to enhance yield and purity for isolating cells from complex tumor tissue.

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Isolation

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