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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

DOI :

10.3791/55157-v

January 19th, 2017

January 19th, 2017

8,270 Views

1Dept. of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, 2The Core Research Facility, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem

We describe here a relatively fast and simple approach for mapping genome-wide mammalian replication timing, from cell isolation to the basic analysis of the sequencing results. A genomic map of a representative replication program will be provided following the protocol.

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