JoVE Journal

Medicine

This content is Open Access.

Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture

A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.

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