JoVE Logo
Faculty Resource Center

Sign In

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

DOI :

10.3791/55637-v

11:39 min

July 21st, 2017

July 21st, 2017

29,571 Views

1Amnis Biology Department, MilliporeSigma

Here, multispectral imaging flow cytometry with an analytical feature that compares bright detail images of 3 autophagy markers and quantifies their co-localization, along with LC3 spot counting, was used to measure autophagy in an objective, quantitative, and statistically robust manner.

Tags

Autophagy Markers

-- Views

Related Videos

article

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

article

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

article

Flow Cytometry Purification of Mouse Meiotic Cells

article

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

article

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

article

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

article

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

article

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

article

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

article

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved