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Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond Video (Video) | JoVE

DOI :

10.3791/50484-v

July 27th, 2013

July 27th, 2013

18,220 Views

1Signalling Programme, The Babraham Institute, 2MRC Group, Cardiff School of Biosciences, Cardiff University

Time-lapse microscopy of fluorescently labeled autophagy markers allows monitoring of the dynamic autophagy response with high temporal resolution. Using specific autophagy and organelle markers in a combination of 3 different colors, we can follow the contribution of a protein to autophagosome formation in a robust spatial and temporal context.

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