JoVE Logo
Faculty Resource Center

Sign In

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

DOI :

10.3791/50101-v

December 26th, 2012

December 26th, 2012

8,661 Views

1Division of Basic Sciences, Fred Hutchinson Cancer Research Center

We present a protocol for freezing and cryosectioning yeast communities to observe internal patterns of fluorescent cells. The method relies on methanol-fixing and OCT-embedding to preserve the spatial distribution of cells without inactivating fluorescent proteins within a community.

-- Views

Related Videos

article

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

article

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

article

Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

article

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

article

Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

article

Direct Infusion Device for Molecule Delivery in Plants

article

Author Spotlight: Marmoset Research - Scope and Challenges

article

Author Spotlight: Advancing Mitochondrial Research – mtHyper7 Biosensor for Subcellular Analysis

article

Author Spotlight: Decoding Corneal Neovascularization with Alkali Burn Model for Future Therapeutic Strategies

article

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved