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Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

DOI :

10.3791/50171-v

March 5th, 2013

March 5th, 2013

34,292 Views

1UCL Cancer Institute, 2Friedrich Miescher Institute for Biomedical Research

A rapid and simple way to generate human cell lines with inducible and reversible cDNA overexpression or shRNA-mediated knock-down of the gene of interest. This method enables researchers to reliably and highly reproducibly manipulate cell lines that are difficult to alter by transient transfection methods or conventional knockdown/knockout strategies.

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