Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
7.7K Views
•
12:19 min
•
February 12th, 2020
DOI :
February 12th, 2020
•Chapters in this video
0:04
Introduction
0:55
C2C12 Transfection
2:35
Transfected C2C12 Selection
3:15
C2C12 Differentiation
3:59
Phenotypic Analysis
6:10
Western Blot Analysis Sample Preparation
9:37
Results: Representative C2C12 Selection, Differentiation, and Knockdown Validation
11:19
Conclusion
Related Videos
06:37
Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
14.7K Views
09:24
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
4.3K Views
10:12
Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing
4.1K Views
09:39
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
15.3K Views
13:54
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
10.9K Views
09:51
Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
35.2K Views
06:12
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
3.4K Views
10:50
Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
949 Views
09:24
Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
9.0K Views
06:49
Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
2.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved