Collecting Worm Population for Gut Microbiome Analyses
2:08
Setting Up the Large Particle Sorter and Autosampler
3:43
Sorting of Caenorhabditis elegans by Gut Microbiome Features
6:05
Results: Profiling Gut Microbiome Colonization
7:50
Conclusion
Transcript
The gut microbiome plays important roles in shaping host's physiology. This protocol, in particular, allows for high throughput assessment of fluorescent gut microbe colonization levels in single, cellular animals on a large scale. One major benef
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Caenorhabditis elegans is a powerful model to examine the molecular determinants driving host-microbiome interactions. We present a high throughput pipeline profiling the single animal levels of gut microbiome colonization together with key aspects of the C. elegans physiology.