Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
11.8K Views
•
11:47 min
•
February 8th, 2013
DOI :
February 8th, 2013
•Rebecca E. Nakles1, Sarah L. Millman1, M. Carla Cabrera1, Peter Johnson1,2, Susette Mueller1,2, Philipp S. Hoppe3, Timm Schroeder3, Priscilla A. Furth1,2,4,5
1Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University
Chapters in this video
0:05
Title
1:48
Generation of Primary Mammary Epithelial Cell Cultures
3:39
Live-cell Imaging
5:56
Assessing Cellular Event from Time-lapse Images
7:50
Making Single Cell Fate Maps with Timm's Tracking Tool (TTT)
9:59
Representative Results: Colony Formation in BRCA1 Disrupted Cells
11:12
Conclusion
Related Videos
06:29
Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland
12.5K Views
10:23
Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
9.0K Views
08:26
Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
24.8K Views
07:45
Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer
5.8K Views
07:44
Longitudinal Intravital Imaging Through Clear Silicone Windows
3.4K Views
07:13
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
23.2K Views
08:57
Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging
2.7K Views
08:11
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
30.6K Views
09:49
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres
12.5K Views
10:53
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
3.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved