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In vitro Organoid Culture of Primary Mouse Colon Tumors

DOI :

10.3791/50210-v

May 17th, 2013

May 17th, 2013

34,231 Views

1Department of Molecular & Integrative Physiology, University of Michigan , 2Department of Internal Medicine, Division of Gastroenterology, University of Michigan

A simple method to establish primary murine colon tumor organoid is described. This method utilizes the feature that colon tumor cells survive and grow into organoids in media containing limited growth factors, whereas normal colon epithelial do not.

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