May 17th, 2013
•A simple method to establish primary murine colon tumor organoid is described. This method utilizes the feature that colon tumor cells survive and grow into organoids in media containing limited growth factors, whereas normal colon epithelial do not.
Tags
Related Videos
Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Primary Culture of Human Vestibular Schwannomas
Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics
In vitro Functional Characterization of Mouse Colorectal Afferent Endings
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved