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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

DOI :

10.3791/60469-v

8:43 min

January 31st, 2020

January 31st, 2020

9,148 Views

1Department of Life Sciences, Pohang University of Science and Technology

This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.

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Tumor Organoids

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